Week 4

This week, we

• learned how to make a cultivation medium for bacteria and plants.✔
• Made 10 Petri Dishes of homemade medium and grew wild bacteria and organisms.✔

How to make medium

medium (/ˈmidiəm/) noun (biology) a substance that something exists or grows in or that it travels through

• The bacteria were growing in a sugar medium. …Oxford Dictionary

We can find various kind of cultivation media here ↓

Cultivation Media

Which media is preferred depends on the organisms.

I chose Nutrient Agar (NA) as my first DIY media!

Nutrient Agar (NA)

Ingredients of Nutrient Agar

• Ingredients for 1000ml of NA

  • Yeast extract 2.0g
  • Peptone 5.0g
  • NaCl 5.0g
  • Agar 15.0g
  • Demi water up to 1000mL

• Ingredients for 250ml of NA

  • yeast extract 0.5g
  • Peptone 1.25g
  • NaCl 1.25g
  • Agar 3.75
  • Demi water

Wild Hunting