Week 6 & 7
This week, we
- tried to understand the CRISPR/Cas9 mechanism, DNA Repair mechanisms: HDR and NHEJ
- Made an account at benchling.com and followed the CRISPR tutorial from Fabio Ferreira on Digital DNA Design. [video]
How to use Benchling?
How To Benchling?
Benchlingを使ってPCRプライマーをデザインする方法 (JP)
(I figured out the way and wrote Japanese version of the instruction, the English version is written by Toh Takuzen).
Understanding CRISPR
CRISPR - How It Works, Top Applications and How to Use It Yourself.
CRISPR_BioHacking_Waag2022.pdf
In-vitro CRISPR