What is PCR?

Polymerase Chain Reaction (PCR) is a technique to amplify the amount of DNA fragments.

→ Inventer: Kary Mullis

PCRとは(基本情報)

The whole process of PCR

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What the PCR method is doing is basically just raising and lowering the temperature. That is why this process is called thermal cycling.

Thermal cycling consists of 3 steps.

  1. Denaturation: 熱変性
  2. Annealing: アニーリング
  3. Extension: 伸長

The first step, denaturation (90℃), will decompose double-stranded DNA into single-stranded DNA